Cosynthesis of l-homophenylalanine and 2-phenylethanol by recombinant Saccharomyces cerevisiae expressing aspartate aminotransferase from Escherichia coli BL21(DE3)

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• 作者：Chunhua Luo ; Qingmei Lin ; Suying Lin ; Chun Meng ; Hang Wang ; ^{wanghang@fzu.edu.cn}
• 关键词：Cosynthesis ; l-Homophenylalanine ; 2-Phenylethanol ; Saccharomyces cerevisiae ; Aspartate aminotransferase
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：123
• 期：1
• 页码：1-7
• 全文大小：1525 K
• 卷排序：123

文摘

The Escherichia coli aspartate aminotransferase gene was introduced into a high 2-phenylethanol (2-PEA) producing Saccharomyces cerevisiae YS58, and the recombinant strain of S. cerevisiae was utilized for the co-production of 2-PEA and l-homophenylalanine (L-HPA) via a fermentation process. The L-HPA productivity of the recombinant S. cerevisiae improved 78.9% in comparison to the wild-type S. cerevisiae. High yields of 43.7 mM L-HPA and 32.4 mM 2-PEA were achieved. As a result, the coupling of the biosynthesis process for these two products in the recombinant strain led to a more complete and efficient utilization of the substrate, l-phenylalanine.