Effect of miR-125b on dermal papilla cells of goat secondary hair follicle
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- 作者：Guangxian Zhou
- 关键词：Anagen ; Antagomir ; Cashmere goats ; Catagen ; Dermal papilla cells ; Hair cycle ; Hair follicle development ; Hair follicle morphogenesis ; Luciferase assay ; MicroRNAs ; Recombinant adenovirus
- 刊名：Electronic Journal of Biotechnology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：25
- 期：Complete
- 页码：64-69
- 全文大小：1035 K
- 卷排序：25
文摘
MicroRNAs (miRNAs) are endogenous noncoding RNAs that regulate various biological processes. miR-125b is a miRNA that has been reported to be critical for hair follicle (HF) morphogenesis and development. We identified that the expression of miR-125b varies during an individual hair cycle (anagen, catagen, and telogen) in the skin of cashmere goats. We constructed a gain model (by overexpressing miR-125b) and a loss model (by inhibiting endogenous miR-125b) based on dermal papilla cells (DPCs) to further investigate the role of miR-125b in HF cycle. In addition, we used a dual-luciferase system to highlight the predicated target genes of miR-125b.ResultsWe found that miR-125b affects the expression of FGF5, IGF-1, SHH, TNF-α, MSX2, LEF-1, FGF7, NOGGIN, BMP2, BMP4, TGF-β1, and β-catenin. The dual-luciferase assay further validated a direct interaction between miR-125b and FGF5 and TNF-α.ConclusionmiR-125b affects the expression levels of genes related to hair cycle and may also play a critical role in regulating the periodic development of HF.