Adsorption of gastric lipase onto multicomponent model lipid monolayers with phase separation
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- 作者：Claire Bourlieua ; claire.bourlieu@rennes.inra.fr" class="auth_mail" title="E-mail the corresponding author ; Gilles Paboeufb ; Sophie Chevera ; Sté ; phane Pezenneca ; Jean-Franç ; ois Cavalierc ; Fanny Guyomarc&rsquo ; ha ; Amé ; lie Deglairea ; Saï ; d Bouhallaba ; Didier Duponta ; Fré ; dé ; ric Carriè ; rec ; Vé ; ronique Vié ; b
- 关键词：AFM ; atomic force microscopy ; DGL ; dog gastric lipase ; DOPC ; Dioleoylphosphatidylcholine ; DPPC ; Dipalmitoylphosphatidylcholine ; DOPE ; Dioleoylphosphatidylethanolamine ; DOPS ; Dioleoylphosphatidylserine ; HGL ; human gastric lipase ; IRS ; interfacial recognition site ; GL ; gastric lipase ; LE ; liquid expanded ; LC ; liquid condensed ; MPL ; milk polar lipids ; PC ; Phosphatidylcholine ; PE ; Phosphatidylethanolamine ; PI ; Phosphatidylinositol ; PS ; Phosphatidylserine ; rDGL ; recombinant dog gastric lipase
- 刊名：Colloids and Surfaces B: Biointerfaces
- 出版年：2016
- 出版时间：1 July 2016
- 年：2016
- 卷：143
- 期：Complete
- 页码：97-106
- 全文大小：3110 K
文摘
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Ellipsometry evidenced high amount of gastric lipase close to interface.
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Gastric lipase adsorption impacts on lipid phase separation.
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Gastric lipase partitions towards LE phase in monolayer with phase coexistence.
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Gastric lipase adsorbs at variable levels of insertion suggesting variable interaction.
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Lipase adsorption is mediated by hydrophobic and electrostatic interactions.