Recombinant anthrax protective antigen: Observation of aggregation phenomena by TEM reveals specific effects of sterols

详细信息    查看全文

• 作者：J. Robin Harris^a ; ^{rharris@uni-mainz.de} ; Andrei Soliakov^b ; Allan Watkinson^c ; Jeremy H. Lakey^d
• 关键词：Anthrax protective antigen ; Aggregation ; Transmission electron microscopy ; Negative staining ; Cholesterol microcrystal ; Sodium deoxycholate ; rPA83 ; PA63 prepore ; PA63 pore
• 刊名：Micron
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：93
• 期：Complete
• 页码：1-8
• 全文大小：3859 K
• 卷排序：93

文摘

rPA83 forms fibrous aggregates when heated at low temperatures (50 °C). rPA83 aggregates binds to the edges of cholesterol bilayer microcrystals. PA63 prepore oligomers bind to the edges of cholesterol bilayer microcrystals. PA63 prepores convert to PA63 pores when treated with sodium deoxycholate. PA63 pores produced by sodium deoxycholate aggregate as dimers and angular chains