Reversible and multi-cyclic protein-protein interaction in bacterial cellulosome-mimic system using rod-shaped viral nanostructure
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- 作者：Hyun Jin Kima ; Eun Jung Leea ; b ; Jin-Seung Parka ; c ; Sang Jun Sima ; Jeewon Leea ; leejw@korea.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cohesin ; Dockerin ; Reversible interaction ; Tobacco mosaic virus coat protein ; Multi-cyclic operation
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 March 2016
- 年：2016
- 卷：221
- 期：Complete
- 页码：101-106
- 全文大小：1908 K
文摘
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The type II cohesin and type II dockerin were cloned from Clostridium thermocellum and expressed in active form with the fusion of TMVcp and EGFP in Escherichia coli.
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We confirmed that the Ca2+-dependent native cohesin-dockerin binding and dissociation between two recombinant fusion proteins, TMVcp-cohesin and EGFP-dockerin.
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The multi-cyclic operation of binding-dissociation between TMVcp-Coh rod and EGFP-dockerin was successfully performed.