Differential effects of the recombinant toxin PnTx4(5-5) from the spider Phoneutria nigriventer on mammalian and insect sodium channels
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- 作者：Ana L.B. Paivaa ; c ; Alessandra Matavela ; Steve Peigneurb ; Marta N. Cordeiroa ; Jan Tytgatb ; Marcelo R.V. Diniza ; Maria Elena de Limac ; lima.mariaelena@gmail.com" class="auth_mail" title="E-mail the corresponding author ; melenalima@icb.ufmg.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Phoneutria nigriventer ; PnTx4(5-5) ; Recombinant toxin ; Insecticide toxin ; Sodium channel
- 刊名：Biochimie
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：121
- 期：Complete
- 页码：326-335
- 全文大小：1228 K
文摘
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We have cloned and heterologously expressed the toxin PnTx4(5-5) in Escherichia coli.
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Secondary structure and biological activity of the recombinant toxin was similar to the native one.
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Toxin strongly slowed down the inactivation of the cockroach sodium channel.
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Toxin inhibited Nav1.2–1.6 mammalian sodium channels.