Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins

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• 作者：Rouhollah Kazemi^a ; ^{kazemir@nigeb.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; Asal Akhavian^a ; ^{a_akhavian@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Jafar Amani^b ; ^{jafar.amani@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jafar Salimian^c ; ^{jafar.salimian@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Mohammad-Javad Motamedi^a ; ^{j.motamedi@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Amir Mousavi^a ; ^{m-amir@nigeb.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; Mahyat Jafari^a ; ^{mahyat_j@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Ali-Hatef Salmanian^a ; ^{salman@nigeb.ac.ir" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Diarrhoea ; ETEC ; EHEC ; Vibrio cholerae ; Recombinant protein ; Subunit vaccine
• 刊名：Microbes and Infection
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：18
• 期：6
• 页码：421-429
• 全文大小：1274 K

文摘

Infectious diarrhoea remains an emerging problem in the world health program. Among diarrheagenic agents, Vibrio cholerae and enterotoxigenic and enterohemorrhagic Escherichia coli are critical enteropathogens. AB5 toxin produced by these bacteria, heat-labile enterotoxin (LT), cholera enterotoxin (CT), and shiga-like cytotoxin (STX) can target the immune system and are subunit vaccine candidates. A chemically-synthesized chimeric construct composed of the binding subunits of these toxins (LTB, STXB, and CTXB) was developed based on bioinformatics studies. The whole chimeric protein (rLSC) and each of the segments (rLTB, rSTXB, and rCTXB) were expressed in a prokaryotic expression system (E. coli), purified, and analysed for their immunogenic properties. The results indicate that these recombinant proteins were effectively able to present appropriate epitopes to an animal model of the immune system which could result in and increase IgG in serum and immune responses that protect against the binding activity of these toxins. The immunological assays revealed that the sera of immunized mice prevented toxins from binding to their specific receptors and neutralized their toxic effects. The proposed construct should be considered as a potent immunogen to prevent toxicity and diarrhoea.