Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library
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- 作者：Priyanka Negia ; priyanka.micro@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Janita Lö ; vgrena ; Pä ; ivi Malmia ; 1 ; Nina Sirkkaa ; Jari Metsob ; Tuomas Huovinena ; 2 ; Eeva-Christine Brockmanna ; Kim Petterssona ; Matti Jauhiainenb ; Urpo Lamminmä ; kia ; urplammi@utu.fi" class="auth_mail" title="E-mail the corresponding author
- 关键词：AP ; alkaline phosphatase ; CAD HDL ; HDL isolated from CAD patients ; ctrl HDL ; HDL isolated from healthy individuals ; rHDL ; reconstituted HDL ; EFI ; europium fluorescence intensifier ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; PC ; phosphatidyl choline ; pNPP ; para-nitrophenylphosphate ; SBTI ; soyabean trypsin inhibitor ; scFv ; single chain variable fragment ; TRF ; time-resolved fluorescence
- 刊名：Clinical Biochemistry
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：49
- 期：6
- 页码：472-479
- 全文大小：1081 K
文摘
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CAD HDL binding antibodies were isolated from synthetic antibody library.
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Several antibodies discriminate between HDL from CAD and healthy subjects.
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Majority of the antibodies were lipid dependent apoA-I binders.
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The isolated antibodies were immunoreactive with plasma HDL.