Application of shRNA-containing herpes simplex virus type 1 (HSV-1)-based gene therapy for HSV-2-induced genital herpes

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• 作者：Zhihong Liu ; Yang Xiang ; Zhun Wei ; Bo Yu ; Yong Shao ; Jie Zhang ; Hong Yang ; Manmei Li ; Ming Guan ; Jun Wan ; Wei Zhang
• 关键词：Herpes simplex virus type 1 (HSV-1) vector ; Herpes simplex virus type 2 (HSV-2) ; Genital herpes
• 刊名：Journal of Virological Methods
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：193
• 期：2
• 页码：353-358
• 全文大小：1249 K

文摘

HSV-1-based vectors have been widely used to achieve targeted delivery of genes into the nervous system. In the current study, we aim to use shRNA-containing HSV-1-based gene delivery system for the therapy of HSV-2 infection. Guinea pigs were infected intravaginally with HSV-2 and scored daily for 100 days for the severity of vaginal disease. HSV-2 shRNA-containing HSV-1 was applied intravaginally daily between 8 and 14 days after HSV-2 challenge. Delivery of HSV-2 shRNA-containing HSV-1 had no effect on the onset of disease and acute virus shedding in animals, but resulted in a significant reduction in both the cumulative recurrent lesion days and the number of days with recurrent disease. Around half of the animals in the HSV-2 shRNA group did not develop recurrent disease 100 days post HSV-2 infection. In conclusion, HSV-2 shRNA-containing HSV-1 particles are effective in reducing the recurrence of genital herpes caused by HSV-2.