Probing the kinetic landscape of Hox transcription factor-DNA binding in live cells by massively parallel Fluorescence Correlation Spectroscopy
详细信息 查看全文
- 作者：Dimitrios K. Papadopoulosa ; 1 ; papadopo@mpi-cbg.de" class="auth_mail" title="E-mail the corresponding author ; Aleksandar J. Krmpotb ; c ; 1 ; Stanko N. Nikolićb ; c ; Robert Krautzd ; Lars Tereniusb ; Pavel Tomancaka ; Rudolf Riglere ; f ; Walter J. Gehringg ; 2 ; Vladana Vukojevićb ; vladana.vukojevic@ki.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hox genes ; Sex combs reduced ; Dimer formation ; Massively parallel Fluorescence Correlation Spectroscopy ; Bimolecular Fluorescence Complementation ; Transcription factor&ndash ; DNA binding
- 刊名：Mechanisms of Development
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：138
- 期：part_P2
- 页码：218-225
- 全文大小：1287 K
文摘
- •
Scr forms dimers predominantly on DNA.
- •
Scr dimers strongly associate with its specific fork head binding site.
- •
The phosphomimicking, inactive variant of Scr, Scr(DD), shows limited dimerization.
- •
Scr shows generally similar concentration but different mobility across the nucleus.
- •
mpFCS allows coincident study of dynamic processes in optical cell/tissue sections.