Spectrophotometric total reducing sugars assay based on cupric reduction
详细信息 查看全文
- 作者：Kevser Sö ; zgen Başkan ; sozgen@istanbul.edu.tr" class="auth_mail" title="E-mail the corresponding author ; Esma Tü ; tem ; Esin Akyü ; z ; Seda Ö ; zen ; Reşat Apak
- 关键词：Reducing sugar ; Spectrophotometry ; Cu(II)-Nc ; DNS ; Glucose ; Fructose
- 刊名：Talanta
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：147
- 期：Complete
- 页码：162-168
- 全文大小：513 K
文摘
- •
Reducing sugar content of specific foods is controlled by European legislation.
- •
A simple spectrophotometric method for reducing sugars was developed.
- •
The reagent is the relatively non-hazardous cupric neocuproine in alkaline medium.
- •
Cu(II)-Nc method is more sensitive and R2≈1 compared to dinitrosalicylate method.
- •
Plant phenolics did not interfere after solid phase extraction in clean-up.