Human dihydrofolate reductase influences the sensitivity of the malaria parasite Plasmodium falciparum to ketotifen - A cautionary tale in screening transgenic parasites

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• 作者：Phuong N. Tran^a ; ¹ ; Cameron J. Tate^a ; Melanie C. Ridgway^a ; Kevin J. Saliba^a ; ^b ; Kiaran Kirk^a ; Alexander G. Maier^a ; ^{alex.maier@anu.edu.au}
• 关键词：hDHFR ; Ketotifen fumarate ; Plasmodium falciparum ; Malaria ; Selectable marker ; Transfection ; Folate
• 刊名：International Journal for Parasitology: Drugs and Drug Resistance
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：6
• 期：3
• 页码：179-183
• 全文大小：928 K
• 卷排序：6

文摘

The use of hDHFR as selectable marker alters sensitivity of P. falciparum to ketotifen. Anti-malarial mechanism of ketotifen is unrelated to expression of parasite gABCG2. hDHFR interacts with ketotifen. Folate concentration used in P. falciparum culture limits parasite growth in vitro.