Biosynthesis of optically pure chiral alcohols by a substrate coupled and biphasic system with a short-chain dehydrogenase from Streptomyces griseus
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- 作者：Zhuotao Tana ; b ; Hongmin Mac ; Qing Lia ; b ; Lingling Pua ; b ; Yang Caoa ; b ; Xudong Quc ; Chenjie Zhua ; b ; d ; zhucj@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Hanjie Yinga ; b ; d ; yinghanjie@njtech.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：SDRs ; short-chain dehydrogenases/reductases superfamily ; CR ; carbonyl reductase ; SgCR ; carbonyl reductase from Streptomyces griseus ; COBE ; ethyl 4-chloro-3-oxobutanoate ; (S)-CHBE ; (S)-4-chloro-3-hydroxybutanoate ; IPT ; gisopropyl β-d-1-thiogalactopy-ranoside ; SDS-PAGE ; sodium dodecyl sulfate⿿polyacrylamide gel electrophoresis ; e.e. % ; enantiomeric excess ; E.coli ; Escherichia coli ; GDH ; glucose dehydrogenase
- 刊名：Enzyme and Microbial Technology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：93-94
- 期：Complete
- 页码：191-199
- 全文大小：2381 K
文摘
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Five carbonyl reductases from different Streptomyces were discovered by genome mining.
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SgCR was characterized, expressed and compared with other SDRs.
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Broad substrate spectrum of SgCR was shown.
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Highly enanioselectivity and conversion of various chiral alcohols were obtained.
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Conditions of bioproducing (S)-CHBE was optimized in a biphasic system.