Highly efficient bioreduction of 2-hydroxyacetophenone to (S)- and (R)-1-phenyl-1,2-ethanediol by two substrate tolerance carbonyl reductases with cofactor regeneration

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• 作者：Zhi-Mei Cui^a ; Jian-Dong Zhang^a ; ^{zhangjiandong@tyut.edu.cn} ; Xiao-Jun Fan^a ; Gao-Wei Zheng^b ; Hong-Hong Chang^a ; ^{changhonghong@tyut.edu.cn} ; Wen-Long Wei^a
• 关键词：Highly enantioselective ; Carbonyl reductase ; 2-Hydroxyacetophenone ; Chiral 1-phenyl-1 ; 2-ethanediol ; Cofactor regeneration
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：243
• 期：Complete
• 页码：1-9
• 全文大小：1436 K
• 卷排序：243

文摘

Two stereocomplementary NADH-dependent carbonyl reductases were discovered for the first time for highly stereoselective bioreduction of 2-HAP to enantiopure PED. Strong substrate tolerance of the two enzymes was demonstrated. Effective cofactor regeneration system was constructed by glucose dehydrogenase, complete conversions were achieved. Biotransformation of 2-HAP (at 54 g/L scale) to enantiopure PED with recombinant E. coli whole cells was achieved without external addition of an expensive cofactor.