Genomic mining-based identification of novel stereospecific aldo-keto reductases toolbox from Candida parapsilosis for highly enantioselective reduction of carbonyl compounds
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- 作者：Rongyun Guoa ; Yao Niea ; ynie@jiangnan.edu.cn" class="auth_mail ; Xiao Qing Mua ; Yan Xua ; b ; yxu@jiangnan.edu.cn" class="auth_mail ; Rong Xiaoc
- 关键词：AKR ; aldo-keto reductase ; ORF ; open reading frame ; IPTG ; isopropyl-尾-d-thiogalactopyranoside ; TCEP ; Tris (2-carboxyethyl) phosphine ; SDS-PAGE ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：July 2014
- 年：2014
- 卷：105
- 期：Complete
- 页码：66-73
- 全文大小：2411 K
文摘
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Aldo-keto reductase (AKR) toolbox was identified from Candida parapsilosis genome.
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The new AKRs were active to various carbonyl compounds with high enantioselectivity.
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An enzyme was gained with excellent enantioselectivity and broad substrate spectrum.
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Genomic mining is powerful to develop novel AKRs for fine biochemical synthesis.