Rational quality assessment procedure for less-investigated herbal medicines: Case of a Congolese antimalarial drug with an analytical report

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• 作者：Dieudonné ; Tshitenge Tshitenge^a ; ^b ; Karine Ndjoko Ioset^a ; José ; Nzunzu Lami^b ; Josaphat Ndelo-di-Phanzu^b ; Jean-Pierre Koy Sita Mufusama^a ; ^b ; Gerhard Bringmann^a ; ^{bringman@chemie.uni-wuerzburg.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：INERA ; Institut National pour l'Etude et la Recherche Agronomiques ; HPLC ; high performance liquid chromatography ; DAD ; diode array detector ; CRS ; chemical reference substance ; ICH ; International Conference on Harmonization ; ANOVA ; analysis of variance ; SD ; standard deviation ; RSD ; repeatability standard deviation ; NMR ; nuclear magnetic resonance ; COSY ; correlation spectroscopy ; TOCSY ; total correlation spectroscopy ; HSQC ; heteronuclear single quantum correlation ; HMBC ; heteronuclear multiple bon
• 刊名：Fitoterapia
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：110
• 期：Complete
• 页码：189-195
• 全文大小：828 K

文摘

Herbal medicines are the most globally used type of medical drugs. Their high cultural acceptability is due to the experienced safety and efficiency over centuries of use. Many of them are still phytochemically less-investigated, and are used without standardization or quality control. Choosing SIROP KILMA, an authorized Congolese antimalarial phytomedicine, as a model case, our study describes an interdisciplinary approach for a rational quality assessment of herbal drugs in general. It combines an authentication step of the herbal remedy prior to any fingerprinting, the isolation of the major constituents, the development and validation of an HPLC-DAD analytical method with internal markers, and the application of the method to several batches of the herbal medicine (here KILMA) thus permitting the establishment of a quantitative fingerprint. From the constitutive plants of KILMA, acteoside, isoacteoside, stachannin A, and pectolinarigenin-7-O-glucoside were isolated, and acteoside was used as the prime marker for the validation of an analytical method. This study contributes to the efforts of the WHO for the establishment of standards enabling the analytical evaluation of herbal materials. Moreover, the paper describes the first phytochemical and analytical report on a marketed Congolese phytomedicine.