Antibodies against neo-epitope tTg complexed to gliadin are different and more reliable then anti-tTg for the diagnosis of pediatric celiac disease

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• 作者：Aaron Lerner^a ; ^b ; ^{aaronlerner1948@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Patricia Jeremias^a ; Sandra Neidhö ; fer^a ; Torsten Matthias^a ; ^{matthias@aesku.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：tTg ; tissue transglutaminase ; tTg-neo ; tTg complexed to gliadin ; PCD ; pediatric celiac disease ; AP ; abdominal pain ; NC ; normal children ; NA ; normal adults ; RA ; rheumatoid arthritis ; CD ; celiac disease ; ESPGHAN ; European Society of Pediatric Gastroenterology ; Hepatology and Nutrition
• 刊名：Journal of Immunological Methods
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：429
• 期：Complete
• 页码：15-20
• 全文大小：735 K

文摘

The neo-epitope tTg (tTg-neo) autoantibody, never challenged the anti-tissue transglutaminase (tTg) premiership, recommended by ESPGHAN, for celiac disease (CD) diagnosis.

Pediatric CD (PCD), abdominal pains and normal children, normal adults, and rheumatoid arthritis patients, were tested using the following ELISAs detecting IgA, IgG or both IgA and IgG (check): AESKULISA® tTg (tTg; RUO) and AESKULISA® tTg-neo.

Higher OD activity was detected for tTg-neo IgA, IgG and IgA + IgG than for tTg. tTg-neo IgA, IgG correlated better with intestinal damage than tTg. The tTg-neo combined IgA + IgG ELISA kit had higher sensitivity and a comparable specificity for the diagnosis of PCD. The drop in the % competition was much higher with the tTg-neo then the tTg antibodies. The false positivity of the tTg was significantly higher than the tTg-neo one.

Serological diagnostic performances, reflection of intestinal damage, diverse epitopes and false positivity were better with the tTg-neo.