Positive rate and score discrepancies of myeloperoxidase staining at different temperatures
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- 作者：Jian Zhong Zhou
- 关键词：AML ; acute myeloid leukemia ; AP ; accelerated phase of chronic myelogenous leukemia ; BP ; blastic phase of chronic myelogenous leukemia ; H2O2 ; hydrogen peroxide ; HR ; hematologic relapse ; MPO ; myeloperoxidase ; M0 ; AML with minimal differentiation ; M1 ; AML without maturation ; M2 ; AML with maturation ; M4 ; acute myelomonocytic leukemia ; M5a ; acute monoblastic leukemia ; M5b ; acute monocytic leukemia ; M5 ; acute monoblastic/monocytic leukemia ; NR ; non-remission ; RAEB ; refractory anemia with excess blasts
- 刊名：Acta Histochemica
- 出版年：January, 2014
- 年：2014
- 卷：116
- 期：1
- 页码：289-291
- 全文大小：287 K
文摘
Myeloperoxidase (MPO) is a strong marker of acute myeloid leukemia. The aim of this study was to compare MPO staining at different temperatures. Four bone marrow aspirate smears of each case were incubated with MPO staining solution at 4 掳C, 20 掳C, 37 掳C and 50 掳C, respectively. 30 patients with score <150 at 50 掳C were group A; 26 patients with score 鈮?50 at 50 掳C were group B. The results showed that at 4 掳C, MPO-positive blasts in two cases of group A were both 2%; at 50 掳C, MPO-positive blasts in the two cases were 42% and 30%, respectively. At 4 掳C, 20 掳C, 37 掳C and 50 掳C, the mean positive rates of group A were 30.6%, 42.2%, 53.7% and 56.5%, respectively (P < 0.001); their mean scores were 52.2, 75.9, 99.5 and 105.3, respectively (P < 0.001); the mean positive rates of group B were 84.1%, 88.7%, 92.3% and 93.7%, respectively (P < 0.001); their mean scores were 199.5, 224.7, 243.5 and 244.5, respectively (P < 0.001). From this study we can conclude that most MPO staining is effective over a wide temperature range with the highest positive rate at 50 掳C. MPO staining intensity at low temperature is much lower and may be false negative. Good MPO staining is achieved at 37 掳C.