Flavonols from Pterogyne nitens and their evaluation as myeloperoxidase inhibitors

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• 作者：Luis Octá ; vio Regasini ; José ; Carlos Rebuglio Vellosa ; Dulce Helena Siqueira Silva ; Maysa Furlan ; Olga Maria Mascarenhas de Oliveira ; Najeh Maissar Khalil ; Iguatemy Lourenç ; o Brunetti ; Mar
• 关键词：Pterogyne nitens ; Fabaceae ; Acylated flavonol ; Myeloperoxidase ; Antioxidant
• 刊名：Phytochemistry
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：69
• 期：8
• 页码：1739-1744
• 全文大小：140 K

文摘

A myeloperoxidase inhibitory kaempferol derivative, namely pterogynoside (1), was isolated from fruits of Pterogyne nitens, along with six known flavonols, kaempferol, afzelin, kaempferitrin, quercetin, isoquercetrin and rutin. The structures of all compounds were elucidated primarily from 1D and 2D NMR spectroscopic analyses, as well as by high resolution mass spectrometry. All flavonols were screened to identify secondary metabolites as potential myeloperoxidase (MPO) inhibitors, and at concentrations of 0.50–50 nM, quercetin (5), isoquercitrin (6) and rutin (7) exhibited strong inhibitory effects with IC₅₀ values of 1.22 ± 0.01, 3.75 ± 0.02 and 3.60 ± 0.02, respectively. The MPO activity detected for the new derivative 1 was markedly decreased (IC₅₀ 10.3 ± 0.03) when compared with known flavonols 5–7, and interestingly increased when tested against ABTS scavenging activity.