Stromal vascular fraction combined with silicone rubber chamber improves sciatic nerve regeneration in diabetes

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• 作者：Rahim Mohammadi^a ; ^{r.mohammadi@urmia.ac.ir" class="auth_mail" title="E-mail the corresponding author} ; Negin Sanaei^a ; Sima Ahsan^a ; Masoume Masoumi-Verki^a ; Fatemeh Khadir^b ; Aram Mokarizadeh^c
• 关键词：Nerve regeneration ; Sciatic nerve ; Silicones ; Diabetes mellitus
• 刊名：Chinese Journal of Traumatology (English Edition)
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：18
• 期：4
• 页码：212-218
• 全文大小：1464 K

文摘

To study the effects of transplantation of characterized uncultured stromal vascular fraction (SVF) on sciatic nerve regeneration.

Methods

A 10-mm sciatic nerve defect was bridged using a silicone conduit filled with SVF. In control group, silicone conduit was filled with phosphate-buffered saline alone. In sham-operated group, the sciatic nerve was only exposed and manipulated. The regenerated nerve fibers were studied 8 and 12 weeks after surgery.

Results

Behavioral and functional studies confirmed faster recovery of regenerated axons in SVF transplanted animals than in control group (p < 0.05). Gastrocnemius muscle mass in SVF transplanted animal was found to be significantly more than that in control group. Morphometric indices of the regenerated fibers showed the number and diameter of the myelinated fibers to be significantly higher in SVF transplanted animals than in control group. In immunohistochemistry, the location of reactions to S-100 in SVF transplanted animals was clearly more positive than that in control group.

Conclusion

SVF transplantation combined with silicone conduit could be considered as a readily accessible source of stromal cells that improves functional recovery of sciatic nerve. It may have clinical implications for the surgical management of acute diabetic patients after facial nerve transection.