In vitro establishment of a highly effective method of castor bean (Ricinus communis L.) regeneration using shoot explants

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• 作者：Ji-xing ZHANG^a ; ^b ; ^{15924475588@163.com" class="auth_mail" title="E-mail the corresponding author} ; Xiao-yu WANG^c ; Zi-zhou FENG^a ; ^d ; Xue-jun GENG^a ; Sha-moli MU^a ; ^b ; Hong-yan HUO^a ; Huan TONG^e ; Meng-zhu LI^f ; Yi LI^f ; Yue CHI^a ; ^b ; Yong-sheng CHEN^a ; ^b ; ^{chenyongshengnmg@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ricinus communis L. ; hypocotyl ; dark pretreatment ; TDZ
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：15
• 期：6
• 页码：1417-1422
• 全文大小：841 K

文摘

An efficient plant regeneration protocol was established for castor bean (Ricinus communis L.), in which 0.3 mg L⁻¹ thidiazuron (TDZ) induced shoot clusters and increased the number of adventitious shoots from hypocotyl tissue. Our results showed that treatment under dark conditions significantly promoted the average number of shoots per explant to 37.36±4.54 (with a 6-d treatment). Modified 1/2 Murashige and Skoog (MS) basal medium supplemented with 440 mg L⁻¹ Ca²⁺, 0.2 mg L⁻¹ gibberellic acid and 0.1 mg L⁻¹ TDZ significantly increased shoot elongation rates and lowered vitrification rates. Furthermore, 1/2 MS media supplemented with 0.2 mg L⁻¹ 1-naphthaleneacetic acid induced a higher rooting rate compared with other culture conditions.