Comparison between pediatric and adult adipose mesenchymal stromal cells

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• 作者：Olivier Abbo¹ ; ² ; Marion Taurand¹ ; Paul Monsarrat¹ ; ³ ; Isabelle Raymond¹ ; ⁴ ; Emmanuelle Arnaud¹ ; Sandra De Barros¹ ; Franç ; oise Auriol³ ; Philippe Galinier³ ; Louis Casteilla¹ ; Valerie Planat-Benard¹ ; ^{valerie.planat@inserm.fr}
• 关键词：adipose mesenchymalstromal cells ; cell therapy ; pediatric cells
• 刊名：Cytotherapy
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：19
• 期：3
• 页码：395-407
• 全文大小：1294 K
• 卷排序：19

文摘

Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications.MethodsSubcutaneous adipose tissue was collected from 27 donors (0–1 years old) and 50 donors (1–12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia.ResultsCells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties.ConclusionThis is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.