Visualisation of collagen fibrils in joint cartilage using STIM

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• 作者：Reinert ; T. ; Reibetanz ; U. ; Vogt ; J. ; Butz ; T. ; Werner ; A. ; Grü ; nder ; W.
• 关键词：07.78 ; 41.75.A ; 61.85 ; 82.78.Ej ; 87.64.F ; Collagen fibrils ; Articular cartilage ; STIM
• 刊名：Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
• 出版年：2001
• 出版时间：July, 2001
• 年：2001
• 卷：181
• 期：1-4
• 页码：511-515
• 全文大小：446 K

文摘

The scanning transmission ion microscopy (STIM) method was used to investigate the collagen network structure of the articular cartilage from a pig's knee in comparison with high resolution nuclear magnetic resonance imaging (microscopic NMR-tomography) and polarised light microscopy (PLM). Single collagen fibrils down to 200 nm in diameter were visualised. It was proved that the cartilage collagen network consists partly of zones of oriented fibrils as suggested by NMR measurements. Radially oriented fibrils were found in the zone near the calcified zone (hypertrophic zone) of both tibia and femur, and in the tibial radial zone. Tangentially oriented fibrils were found in the femoral and tibial superficial zone and in a second zone of the femoral cartilage. Polarisation light microscopy reveals broader zones of orientation than it was found with STIM.