Spatially resolved elemental distributions in articular cartilage
详细信息 查看全文
- 作者：Reinert ; T. ; Reibetanz ; U. ; Vogt ; J. ; Butz ; T. ; Werner ; A. ; Grü ; nder ; W.
- 关键词：07.78 ; 41.75.A ; 61.85 ; 82.78.Ej ; 87.64.F ; Hydrogen analysis ; Elemental analysis ; Micro-PIXE ; Articular cartilage ; Arthrosis
- 刊名：Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
- 出版年：2001
- 出版时间：July, 2001
- 年：2001
- 卷：181
- 期：1-4
- 页码：516-521
- 全文大小：353 K
文摘
In this study, the nuclear microprobe technique is employed to analyse the chemistry of joint cartilage in order to correlate internal structures of the collagen network with the elemental distribution. The samples were taken from pig's knee joint. 30 μm thick coronar cross-sections were prepared by means of cryosectioning and freeze-drying. We performed simultaneously particle induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and elastic recoil detection analysis (ERDA). Thus we obtained spatially resolved distributions of the elements H, C, N, O, P, S, Cl, K and Ca. The main components of the organic matrix are H, C, N and O. It was shown that their relations vary with the cartilage structures. It could be shown that zones with aligned collagen fibrils contain less sulphur and potassium but more chlorine. The higher chlorine concentration is remarkable because newest biochemical studies found that hypochloric acid is involved in cartilage degradation. Furthermore, the calcium distribution is still of great interest. Its correlation to structural changes inside the cartilage is still being discussed. It could be disproved that zones of higher calcium concentration are related to the aligned structures of the collagen network.