BM-40 and MMP-2 expression are not coregulated in human melanoma cell lines

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• 作者：Nischt ; Roswitha ; Wallich ; Martina ; Reibetanz ; Marion ; Baumann ; Petra ; Krieg ; Thomas ; Mauch ; Cornelia
• 关键词：Melanoma cell ; Extracellular matrix protein ; SPARC/BM-40/osteonectin ; Matrix metalloproteinase-2
• 刊名：Cancer Letters
• 出版年：2001
• 出版时间：January 26, 2001
• 年：2001
• 卷：162
• 期：2
• 页码：223-230
• 全文大小：215 K

文摘

As published recently, suppression of BM-40 (osteonectin, SPARC) expression reduced the in vitro adhesive and invasive capacities of melanoma cells. In parallel, a decreased production of matrix metalloproteinase-2 (MMP-2) was observed in these cells indicating a close coregulation of these two proteins. However, analysis of 12 independently established melanoma cell lines, exhibiting different metastatic capacities, did not reveal a positive correlation between BM-40 or MMP-2 expression and the metastatic capacities of these cell lines. In addition, direct comparison of BM-40 and MMP-2 mRNA levels did not provide evidence for coregulation of these two proteins. This was further corroborated by the finding that exogenous BM-40 failed to alter synthesis and activation of proMMP-2.