Protein incorporation into MALDI-matrix crystals investigated by high resolution field emission scanning electron microscopy
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- 作者：Horneffer ; Verena ; Reichelt ; Rudolf ; Strupat ; Kerstin
- 关键词：Matrix&ndash ; analyte interaction ; MALDI-mass spectrometry ; Scanning electron microscopy ; Gold label
- 刊名：International Journal of Mass Spectrometry
- 出版年：2003
- 出版时间：March 15, 2003
- 年：2003
- 卷：226
- 期：1
- 页码：117-131
- 全文大小：528 K
文摘
In this study, high resolution field emission scanning electron microscopy (FE-SEM) was tested for its feasibility to investigate analyte incorporation into and analyte distribution in slowly grown crystals of 2,5-dihydroxybenzoic acid (2,5-DHB) and 2,6-dihydroxybenzoic acid (2,6-DHB); both compounds function as a matrix for matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). To investigate matrix–analyte interaction in 2,5-DHB crystals two Au-labels (20nm colloidal gold and Nanogold) were examined as potential protein markers to visualize proteins in matrix solids by SEM. For this purpose, analyte-doped 2,5-DHB crystals were mechanically cleaved and SEM-micrographs of the opened inner faces were recorded.