The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: Target proteins identified

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• 作者：Reinhard Depping ; Christiane Lohaus ; Helmut E. Meyer and Wolfgang Rü ; ger
• 关键词：ADP-ribosyltransferase ; Bacteriophage T4 ; Alt ; ModB ; Trigger-factor
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2005
• 出版时间：2005
• 年：2005
• 卷：335
• 期：4
• 页码：1217-1223
• 全文大小：196 K

文摘

Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total 27) and ModB (total 8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.