Synergistic cooperation of Sall4 and Cyclin D1 in transcriptional repression

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• 作者：Johann Bö ; hm ; Frank J. Kaiser ; Wiktor Borozdin ; Reinhard Depping ; Jü ; rgen Kohlhase
• 关键词：SALL4 ; Okihiro syndrome ; Zinc finger ; Nuclear localization ; Cyclin D1 ; Cell cycle regulation ; Transcriptional activity
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：11 May 2007
• 年：2007
• 卷：356
• 期：3
• 页码：773-779
• 全文大小：968 K

文摘

Loss of function mutations in SALL4 cause Okihiro syndrome, an autosomal dominant disorder characterised by radial ray malformations associated with Duane anomaly. In zebrafish and mouse Sall4 interacts with TBX5 during limb and heart development and plays a crucial role for embryonic stem (ES) cell pluripotency. Here we report the nuclear interaction of murine Sall4 with Cyclin D1, one of the main regulators of G₁ to S phase transition in cell cycle, verified by yeast two-hybrid assay, co-immunoprecipitation and intracellular co-localisation. Furthermore, using luciferase reporter gene assays we demonstrate that Sall4 operates as a transcriptional repressor located to heterochromatin and that this activity is modulated by Cyclin D1.