DNA cytosine hydroxymethylation levels are distinct among non-overlapping classes of peripheral blood leukocytes
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- 作者：Natalie M. Hohosa ; nhohos@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Kevin Leeb ; klee84@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Lexiang Jic ; lxji@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Miao Yud ; miaoyu1988@uchicago.edu" class="auth_mail" title="E-mail the corresponding author ; Muthugapatti M. Kandasamyb ; kandu@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Bradley G. Phillipse ; bgp@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Clifton A. Bailea ; 1 ; Chuan Hed ; chuanhe@uchicago.edu" class="auth_mail" title="E-mail the corresponding author ; Robert J. Schmitzb ; schmitz@uga.edu" class="auth_mail" title="E-mail the corresponding author ; Richard B. Meagherb ; meagher@uga.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ACTB ; cytoplasmic beta actin ; DNMT1 ; 3A ; 3B ; DNA methyltransferase 1 ; 3A ; 3B ; CG ; cytosine-guanine dinucleotide ; CNS ; conserved noncoding sequence ; CT ; cycle threshold ; GADD45A ; B ; G ; Growth arrest DNA damage inducible proteins 45a ; 45b ; 45g ; 5hmC ; 5&prime ; -hydroxymethylcytosine ; GO ; gene ontology ; 5hmCG ; 5-hydroxymethylcytosine-guanine dinucleotide ; IFM ; Immuno-Fluorescence Microscopy ; 5mC ; 5&prime ; -methylcytosine ; 5mCG ; 5&prime ; -methyl CG dinucleotide ; MBD2 ; MBD4 ; MeCP2 ; Methyl-CpG Binding Dom
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：436
- 期：Complete
- 页码：1-15
- 全文大小：2007 K
文摘
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Reiterative isolation of several non-overlapping leukocyte types from fresh or frozen blood
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Leukocytes suitable for cell type specific epigenetic analysis
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Classes of peripheral leukocytes distinctly regulate factors for the synthesis and removal of 5mCG.
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Classes of peripheral leukocytes are distinct in their levels and distribution of 5hmCG.
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CD4 +, CD8 + and CD14 + cells appear to be poised to respond via changes in 5mCG.