Efficacy of exogenous hormone (GnRHa) for induced breeding of climbing perch Anabas testudineus (Bloch, 1792) and influence of operational sex ratio on spawning success

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• 作者：Babita Mandal^a ; Rajesh Kumar^b ; ^{rajeshfishco@yahoo.co.uk" class="auth_mail" title="E-mail the corresponding author} ; P. Jayasankar^b
• 关键词：Induced breeding ; Spawning success ; Operational sex ratio ; Climbing perch ; Anabas testudineus ; Hormone dose
• 刊名：Animal Reproduction Science
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：171
• 期：Complete
• 页码：114-120
• 全文大小：1507 K

文摘

The climbing perch, Anabas testudineus, is an air-breathing fish having great consumer preference as a food fish and is considered a prime candidate species for aquaculture. Spawning success is an important issue while using hormones for captive induced breeding. In the first experiment, a trial was conducted to assess the efficacy of a synthetic Gonadotropin Releasing Hormone analog (sGnRHa) on the spawning success of climbing perch. Female fish were administered six different doses each with a single intramuscular injection of sGnRHa hormone at 0.002 (T_OD1), 0.005 (T_OD2), 0.01 (T_OD3), 0.015 (T_OD4), 0.02 (T_OD5), 0.03 (T_OD6) μg/g body weight. Similarly, males were administered half of the hormone dose of females in all the respective treatment groups. The greatest (P < 0.05) relative fecundity (715.13 ± 15.0 eggs/g female body weight) and fertilization percentage rates (93.1 ± 8.0%) occurred when female fish were treated at the 0.015 μg/g body weight dose. There was a reduction in relative fecundity and hatching rate in female fish injected with the largest dose (1.5 μL/g body weight) of sGnRHa. A second experiment was conducted to assess the effect of a different male-female ratio on optimum spawning success in climbing perch. For this study a different female to male ratio (1:1, 1:2, 1:3 and 1:4) and male to female ratio (1:1, 1:2 and 1:3) were used. There were a greater (P < 0.05) relative fecundity (886.62 ± 17.9 eggs/g female body weight), fertilization (98 ± 6.7%) and hatching (99 ± 5.4%) rates with the female to male ratio of 1:2. This indicated that the hormone dose of 0.015 μg/g body weight and a female-male ratio of 1:2 are optimal for enhanced spawning success in the climbing perch.