RNA-Seq analysis of immune-relevant genes in Lateolabrax japonicus during Vibrio anguillarum infection
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- 作者：Chao Zhaoa ; c ; 1 ; Mingjun Fua ; c ; 1 ; Chengyang Wanga ; b ; c ; Zongyao Jiaoe ; ; Lihua Qiua ; c ; d ; qiu902@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lateolabrax japonicus ; Vibrio anguillarum ; RNA-Seq ; Immune-relevant genes ; Differentially expressed genes
- 刊名：Fish & Shellfish Immunology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：52
- 期：Complete
- 页码：57-64
- 全文大小：871 K
文摘
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In this study, the widely analysis at the transcriptome level for Lateolabrax japonicus that infected with Vibrio anguillarum was performed.
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In total, 334,388,688 high quality reads were obtained, and de novo assembled into 101,860 Unigenes. Based on sequence similarity 30,142 unigenes were annotated in the public databases.
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In total 107 DEGs related to immunity were screened for further study.
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In total 16 genes were randomly chosen for qRT-PCR analysis, the trend in qRT-PCR expression was in agreement with the RNA-Seq expression profile.