AHSA1 regulates proliferation, apoptosis, migration, and invasion of osteosarcoma

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• 作者：Jianli Shao^a ; ¹ ; Liang Wang^b ; ^c ; ¹ ; ^{wangliangmd@126.com" class="auth_mail" title="E-mail the corresponding author} ; Cheng Zhong^d ; ^e ; ¹ ; Renbin Qi^c ; Yuming Li^d ; ^e ; ^{wyzyykjk@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AHSA1 ; Osteosarcoma ; Cell growth ; Migration ; Invasion
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：77
• 期：Complete
• 页码：45-51
• 全文大小：1603 K

文摘

Activator of 90 kDa heat shock protein ATPase homolog 1 (AHSA1) is a chaperone of heat shock 90 kDa (HSP90) and stimulates ATPase activity of HSP90. The function of AHSA1 in osteosarcoma (OS) has not been reported yet. A previous study showed AHSA1 was overexpressed in OS cells. In this study, we investigated the role of AHSA1 in OS cells by silencing AHSA1. We report that silencing AHSA1 inhibited cell growth, migration, and invasion, and increased apoptosis of MG-63 and Saos2 cells. We also found that silencing AHSA1 decreased the ATPase activity of HSP90 in OS cells. In addition, silencing AHSA1 increased the levels of negative regulators of Wnt/β-catenin signalling pathway, Axin-2 and GSK3β, and decreased the levels of two key members of Wnt/β-catenin signalling pathway, namely, Wnt-5a and β-catenin. In conclusion, silencing AHSA1 regulates cell growth, apoptosis, migration, and invasion by regulating Wnt/β-catenin signalling pathway and their negative regulators.