Suppression of hepatitis B virus antigen production and replication by wild-type HBV dependently replicating HBV shRNA vectors in vitro and in vivo
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- 作者：Baosheng Lia ; b ; 1 ; Shuo Sunb ; c ; 1 ; Minran Lib ; d ; 1 ; Xin Chengb ; Haijun Lib ; Fubiao Kangb ; Jiwen Kangb ; Katharina Dö ; rnbracke ; Michael Nassale ; 1 ; nassal2@ukl.uni-freiburg.de" class="auth_mail" title="E-mail the corresponding author ; Dianxing Sunb ; 1 ; sundianxing@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Hepatitis B virus ; HBV vector ; RNAi ; Chronic hepatitis B ; HBV transgenic mice ; shRNA delivery
- 刊名：Antiviral Research
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：134
- 期：Complete
- 页码：117-129
- 全文大小：3196 K
文摘
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Wild-type HBV dependently replicating HBV shRNA vectors are proposed as self-renewing, self-limiting anti-HBV RNAi agents.
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Optimized HBV vectors encoding two shRNAs replicated in vitro when complemented by HBV helper or wild-type HBV constructs.
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HBV vectors efficiently silenced a model target as well as wild-type HBV antigen production and replication in vitro.
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HBV shRNA vectors delivered into HBV transgenic mice by an adenovirus shuttle significantly reduced serum HBsAg and HBV load.
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Vector treated mice produced progeny vector particles but proving spread will require a small animal infection model.