The early UL3 gene of equine herpesvirus-1 encodes a tegument protein not essential for replication or virulence in the mouse

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• 作者：Byung Chul  ; Ahn^a ; Seongman  ; Kim^a ; Yunfei  ; Zhang^a ; Robert A.  ; Charvat^a ; Dennis J.  ; O'Callaghan ;   ; ^a ;   ; ^{docall@lsuhsc.edu}
• 关键词：Equine herpesvirus 1 ; UL3 gene of EHV-1 ; Tegument protein of EHV-1 ; UL3 ; not essential for EHV-1 virulence
• 刊名：Virology
• 出版年：2011
• 出版时间：10 November 2011
• 年：2011
• 卷：420
• 期：1
• 页码：20-31
• 全文大小：1658 K

文摘

The UL3 gene of equine herpesvirus-1 (EHV-1) is retained in the genome of defective interfering particles and encodes a ~ 33 kDa myristylated protein. Further characterization showed that the UL3 gene is trans-activated only by the sole immediate early (IE) protein and encodes an early protein that is dispensable for EHV-1 replication and localizes in the tegument of purified virions. UL3-deleted EHV-1 (vL11¦¤UL3) exhibits properties of host cell tropism, plaque size, and growth kinetics similar to those of the parental virus. Expression levels of EHV-1 proteins representative of all three gene classes in vL11¦¤UL3-infected cells were identical to those in cells infected with parental virus. Mice intranasally infected with vL11¦¤UL3 and parental virus showed no significant difference in mortality or virus lung titers. These findings suggest that the UL3 protein does not play a major role in the biology of EHV-1 in cell culture or virulence in the mouse.