cis-Prenyltransferase AtCPT6 produces a family of very short-chain polyisoprenoids in planta

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• 作者：Liliana Surmacz ; Danuta Plochocka ; Magdalena Kania ; Witold Danikiewicz ; Ewa Swiezewska
• 关键词：AtCPT ; Arabidopsis thaliana cis-prenyltransferase ; HPLC ; High Performance Liquid Chromatography ; IPP ; isopentenyl diphosphate ; i.u. ; isoprenoid unit ; Pren/Dol-n ; prenol/dolichol composed of n i.u.
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：February, 2014
• 年：2014
• 卷：1841
• 期：2
• 页码：240-250
• 全文大小：1594 K

文摘

cis-Prenyltransferases (CPTs) comprise numerous enzymes synthesizing isoprenoid hydrocarbon skeleton with isoprenoid units in the cis (Z) configuration. The chain-length specificity of a particular plant CPT is in most cases unknown despite the composition of the accumulated isoprenoids in the tissue of interest being well established. In this report AtCPT6, one of the nine Arabidopsis thaliana CPTs, is shown to catalyze the synthesis of a family of very short-chain polyisoprenoid alcohols of six, seven, and eight isoprenoid units, those of seven units dominating. The product specificity of AtCPT6 was established in vivo following its expression in the heterologous system of the yeast Saccharomyces cerevisiae and was confirmed by the absence of specific products in AtCPT6 T-DNA insertion mutants and their overaccumulation in AtCPT6-overexpressing plants. These observations are additionally validated in silico using an AtCPT6 model obtained by homology modeling. AtCPT6 only partially complements the function of the yeast homologue of CPT-Rer2 since it restores the growth but not protein glycosylation in rer2螖 yeast. This is the first in planta characterization of specific products of a plant CPT producing polyisoprenoids. Their distribution suggests that a joint activity of several CPTs is required to produce the complex mixture of polyisoprenoid alcohols found in Arabidopsis roots.