Screening of polymeric supports and enzymes for the development of an endo enzyme cleavable linker

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• 作者：Ana Chiva ; David E. Williams ; Alethea B. Tabor ; Helen C. Hailes
• 关键词：Esterases ; Lipases ; Enzyme cleavable linkers ; Solid-phase reactions ; LPOS
• 刊名：Tetrahedron Letters
• 出版年：2010
• 出版时间：19 May 2010
• 年：2010
• 卷：51
• 期：20
• 页码：2720-2723
• 全文大小：322 K

文摘

Several polymeric supports possessing an ester moiety were prepared and a range of enzymes was investigated to hydrolyse the ester linkage and release a signalling group into solution for applications in immunoassays. Pseudomonas lipases were found to most readily cleave the solution-phase analogue and this observation translated well to the corresponding polymeric supports, where the most effective were PEGA resins and the LPOS support PEG-6000.