文摘
Target-site resistance of Tetranychus urticae to METI-I acaricides, inhibiting mitochondrial complex I, was investigated. A H92R substitution was identified in the PSST homologue of complex I from T. urticae in METI-I acaricide resistant strains. 3D-modeling revealed that H92R is located near the presumed binding site of ubiquinone and well-known complex I inhibitors. Genetic mapping and QTL analysis identified the genomic locus of pyridaben resistance, which included the PSST gene. Selection experiments and marker-assisted back-crossing showed that H92R is associated with METI-I resistance in T. urticae.