Molecular basis of polyspecificity of the Small Multidrug Resistance Efflux Pump AbeS from Acinetobacter baumannii
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- 作者：Iryna Lytvynenko1 ; &dagger ; ; Shlomo Brill2 ; &dagger ; ; Christine Oswald1 ; 3 ; Klaas M. Pos1 ; pos@em.uni-frankfurt.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：CCCP ; carbonylcyanid-m-chlorophenylhydrazone ; DDM ; n-dodecyl-β-d-maltoside ; MPP+ ; 1-methyl-4-phenylpyridinium ; MV2 ; + ; methyl viologen ; Ni-NTA ; Ni2 ; +-nitrilotriacetic acid ; RND ; resistance nodulation and cell division ; SMR ; small multidrug resistance ; TPP+ ; tetraphenylphosphonium
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：13 February 2016
- 年：2016
- 卷：428
- 期：3
- 页码：644-657
- 全文大小：1124 K
文摘
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We purified and reconstituted AbeS, and we analyzed its drug efflux properties.
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Transport of drugs occurs via an electrogenic nH+/drug+ antiport mechanism.
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Size and planarity of aromatic moieties determine substrate recognition.
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The A16G AbeS variant displays a substrate-specific hyperactive phenotype.