Bone marrow stroma cells are susceptible to prion infection

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• 作者：Yuka Takakura ; Naohiro Yamaguchi ; Takehiro Nakagaki ; Katsuya Satoh ; Jun-ichi Kira ; Noriyuki Nishida
• 关键词：Prion protein ; CJD ; BSE ; Bone marrow stromal cell ; MSC
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2008
• 出版时间：19 December 2008
• 年：2008
• 卷：377
• 期：3
• 页码：957-961
• 全文大小：307 K

文摘

Abnormal protease-resistant prion protein (PrP-res) is the only surrogate biochemical marker for prion diseases, and a sensitive technique to detect PrP-res in blood or tissues is urgently needed. Primary cultured bone marrow stromal cells (MSCs) expressed PrP and were capable of supporting stable human prion infection. Using a mouse-adapted BSE strain, we demonstrated that PrP-res can be detected in expanded MSCs. We then analyzed the bone marrow cells collected at autopsy from two individuals with sporadic Creutzfeldt-Jakob disease (CJD), and, in both cases, cultured MSCs were positive for PrP-res. These data would suggest that ex vivo MSC expansion accompanied by PrP-res analysis could be a helpful tool in the definitive diagnosis of prion disease at an earlier stage in the disease process than is currently possible, and with considerably less distress to the patient.