Identification of CCL2, RARRES2 and EFNB2 as host cell factors that influence the multistep replication of respiratory syncytial virus
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- 作者：Kazuya Shiratoa ; shirato@nih.go.jp" class="auth_mail" title="E-mail the corresponding author ; Makoto Ujikeb ; Miyuki Kawasea ; Shutoku Matsuyamaa
- 关键词：CCL2 ; chemokine (C&ndash ; C motif) ligand 2 ; DMEM ; Dulbecco&rsquo ; s modified Eagle&rsquo ; s medium ; EFNB2 ; ephrin-B2 ; FCS ; foetal calf serum ; IFA ; immunofluorescence assay ; PFU ; plaque-forming unit ; RARRES2 ; retinoic acid receptor responder protein 2 ; RSV ; respiratory syncytial virus ; RT-PCR ; reverse transcription polymerase chain reaction ; WB ; Western blot
- 刊名：Virus Research
- 出版年：2015
- 出版时间：2 December 2015
- 年：2015
- 卷：210
- 期：Complete
- 页码：213-226
- 全文大小：2975 K
文摘
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MDCK cells had reduced susceptibility at a low m.o.i. RSV infection.
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RSV-susceptible MDCK cells were obtained by transfection of cDNA libraries.
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CCL2, RARRES2, and EFNB2 were identified by microarray analysis.
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Expression and knockdown of identified genes affected RSV replication and spread.