Glutamate dehydrogenase isoenzyme 3 (GDH3) of Arabidopsis thaliana is regulated by a combined effect of nitrogen and cytokinin

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• 作者：Laura Marchi ; Francesca Degola ; Eugenia Polverini ; Th茅r猫se Terc茅-Laforgue ; Fr茅d茅ric Dubois ; Bertr ; Hirel ; Francesco Maria Restivo
• 关键词：Glutamate dehydrogenase ; Isoenzymes ; Subunits ; Nitrogen starvation ; Cytokinin
• 刊名：Plant Physiology and Biochemistry
• 出版年：December, 2013
• 年：2013
• 卷：73
• 期：Complete
• 页码：368-374
• 全文大小：1022 K

文摘

In higher plants, NAD(H)-glutamate dehydrogenase (GDH; EC 1.4.1.2) is an abundant enzyme that exists in different isoenzymic forms. In Arabidopsis thaliana, three genes (Gdh1, Gdh2 and Gdh3) encode three different GDH subunits (尾, 伪 and 纬) that randomly associate to form a complex array of homo- and heterohexamers. The modification of the GDH isoenzyme pattern and its regulation was studied during the development of A. thaliana in the gdh1, gdh2 single mutants and the gdh1-2 double mutant, with particular emphasis on GDH3. Investigations showed that the GDH3 isoenzyme could not be detected in closely related Arabidopsis species. The induction and regulation of GDH3 activity in the leaves and roots was investigated following nitrogen deprivation in the presence or absence of sucrose or kinetin. These experiments indicate that GDH3 is likely to play an important role during senescence and nutrient remobilization.