Soluble expression and enzymatic activity evaluation of protease from reticuloendotheliosis virus
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- 作者：Feng Hua ; 1 ; hufengggg@126.com" class="auth_mail" title="E-mail the corresponding author ; Yan Zhaoa ; 1 ; kriyazhao@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaole Qia ; qxl@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Hongyu Cuia ; cuihongyu@caas.cn" class="auth_mail" title="E-mail the corresponding author ; Yulong Gaoa ; ylg@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Honglei Gaoa ; ghl@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Changjun Liua ; liucj93711@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Yongqiang Wanga ; yqw_19@yahoo.cn" class="auth_mail" title="E-mail the corresponding author ; Yanping Zhanga ; zhangyanping03@caas.cn" class="auth_mail" title="E-mail the corresponding author ; Kai Lia ; likaihvri@163.com" class="auth_mail" title="E-mail the corresponding author ; Xiaomei Wanga ; b ; xmw@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Yunfeng Wanga ; c ; yfwang@hvri.ac.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Reticuloendotheliosis virus ; Protease ; Expression ; Purification ; Enzyme activity
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：114
- 期：Complete
- 页码：64-70
- 全文大小：873 K
文摘
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Expression of REV PR requires codon-optimization.
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REV PR was expressed in soluble form and purified efficiently.
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The enzymatic activity of PR was analyzed using the substrate of p18-p30.
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The optimal enzymatic reaction system in vitro was preliminarily developed.
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The residue Thr (amino acids 28) plays key role for the enzymatic activity of PR.