Performance of the CRAFTi portable fluorometer comparing with the HPLC method for determining serum retinol

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• 作者：Laksana Chaimongkol^a ; ^b ; Siwaporn Pinkaew^b ; Harold C. Furr^a ; ^c ; John Estes^d ; Neal E. Craft^e ; Emorn Wasantwisut^a ; Pattanee Winichagoon^a ; ^{nupwn@mahidol.ac.th"" rel=""nofollow} ; ^{pattaneew@gmail.com"" rel=""nofollow}
• 关键词：Vitamin A ; Retinol ; Nutritional status ; Fluorometry
• 刊名：Clinical Biochemistry
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：44
• 期：12
• 页码：1030-1032
• 全文大小：187 K

文摘

Objective

To determine the performance of a portable fluorometer for measuring serum retinol (SR) concentration.

Design and methods

Serum samples were obtained from 75 factory worker women and 143 school children. SR concentration was quantified using a portable fluorometer (‘CRAFTi’) and HPLC analysis.

Results

SR by HPLC (1.23 ± 0.43 μmol/L) and CRAFTi (1.16 ± 0.46 μmol/L) was significantly correlated. Sensitivity and specificity were 85.3 % and 78.0 % (cutoff of 1.05 μmol/L). Kappa statistics showed moderate agreement.

Conclusions

CRAFTi portable fluorometer is a promising field-friendly tool for screening vitamin A deficiency.