NADPH oxidase-4 and MATER expressions in granulosa cells: Relationships with ovarian aging

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• 作者：Tullia Maraldi^a ; ^{tullia.maraldi@unimore.it" class="auth_mail" title="E-mail the corresponding author} ; Elisa Resca^b ; ^{elisa.resca@unimore.it" class="auth_mail" title="E-mail the corresponding author} ; Alessia Nicoli^c ; ^{nicoli.alessia@asmn.re.it" class="auth_mail" title="E-mail the corresponding author} ; Francesca Beretti^a ; ^{francesca.beretti@unimore.it" class="auth_mail" title="E-mail the corresponding author} ; Manuela Zavatti^a ; ^{manuela.zavatti@unimore.it" class="auth_mail" title="E-mail the corresponding author} ; Francesco Capodanno^c ; ^{capodanno.francesco@asmn.re.it" class="auth_mail" title="E-mail the corresponding author} ; Daria Morini^c ; ^{morini.daria@asmn.re.it" class="auth_mail" title="E-mail the corresponding author} ; Stefano Palomba^c ; ^{palomba.stefano@asmn.re.it" class="auth_mail" title="E-mail the corresponding author} ; Giovanni B. La Sala^c ; ^d ; ^{lasala.giovanni@asmn.re.it" class="auth_mail" title="E-mail the corresponding author} ; Anto De Pol^a ; ^{anto.depol@unimore.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：MATER ; NOX4 ; Granulosa cells ; Infertility
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：1 October 2016
• 年：2016
• 卷：162
• 期：Complete
• 页码：108-114
• 全文大小：859 K

文摘

Relevant roles in follicular development and ovulation are played by maternal antigen that embryos require (MATER), product of a maternal effect gene, and by reactive oxygen species (ROS), indispensable for the induction of ovulatory genes. At the moment, the relationship between these two biological systems and their involvement in the ovarian aging have not been still clarified. The aim of the current experimental study was to analyse the age-related changes of the MATER and NOX proteins.

Materials and methods

MATER and ROS homeostasis was studied in granulosa cells (GCs) and cumulus cells (CCs) of infertile patients who undergone oocyte retrieval for in vitro fertilization cycles using Western blot and confocal immunofluorescence analysis. Samples were obtained from subjects with age ≥ 40 years (cases) and with age ≤ 37 years (controls).

Key findings

The expression pattern of MATER and NOX observed in GCs was not different from that observed in CCs. High levels of both proteins were detected in the control samples. A significant lower expression of both MATER and NOX4 was observed in the case versus control samples.

Significance

The expression of MATER and NOX4 proteins are closely related to the follicular development and ovulation with particular regard for ovarian aging.