A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene
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- 作者：Gilles Gadea ; Sandra Bos ; Pascale Krejbich-Trotot ; Elodie Clain ; Wildriss Viranaicken ; Chaker El-Kalamouni ; Patrick Mavingui ; Philippe Desprè ; s ; philippe.despres@univ-reunion.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Arbovirus ; Emerging disease ; Flavivirus ; Zika virus ; Molecular clones ; Recombinant virus ; GFP reporter
- 刊名：Virology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：497
- 期：Complete
- 页码：157-162
- 全文大小：1362 K
文摘
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The reverse genetic method ISA is a convenient method for rapid production of ZIKV molecular clones.
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Molecular clones are reliable tools for investigation on ZIKV viral determinants of human disease.
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The ISA method allows the rapid production of recombinant ZIKVGFP expressing the GFP reporter gene.
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ZIKVGFP is suitable for dynamics study on viral replication in host cells from mosquito to human.
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ZIKVGFP is suitable to test antiviral activity of inhibitors as well as of neutralizing antibodies.