Spliced leader-based analyses reveal the effects of polycyclic aromatic hydrocarbons on gene expression in the copepod Pseudodiaptomus poplesia

详细信息    查看全文

• 作者：Yunyun Zhuang^a ; ¹ ; Feifei Yang^a ; ^b ; ¹ ; Donghui Xu^a ; ² ; Hongju Chen^a ; Huan Zhang^a ; ^c ; ^{huan.zhang@uconn.edu} ; ^{huan.zhang2@gmail.com} ; Guangxing Liu^a ; ^{gxliu@ouc.edu.cn}
• 关键词：1 ; 2 NAPH ; 1 ; 2-dimethylnaphthalene ; PAHs ; Polycyclic aromatic hydrocarbons ; RACE ; Rapid amplification of cDNA ends ; RT-qPCR ; reverse-transcription quantitative PCR ; SL ; Spliced leader
• 刊名：Aquatic Toxicology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：183
• 期：Complete
• 页码：114-126
• 全文大小：2655 K
• 卷排序：183

文摘

Pseudodiaptomus poplesia was exposed to 1,2-dimethylnaphthalene (1,2-NAPH) and pyrene. The 96-h LC50 of 1, 2-NAPH and pyrene were 788.98 μg/L and 54.68 μg/L, respectively. Spliced leader-based cDNA libraries revealed genes responding to PAHs exposure. Reference genes for RT-qPCR were evaluated for P. poplesia exposed the two PAHs. Changes in expression of seven genes revealed the toxicity of PAHs may be linked to oxidative stress.