Substitutions in position 222 of haemagglutinin of pandemic influenza A (H1N1) 2009 viruses in Spain

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• 作者：Juan Ledesma^a ; ^{jledesmam@isciii.es} ; Francisco Pozo^a ; Mercedes Pé ; rez Ruiz^b ; Jose Marí ; a Navarro^b ; Luis Pi&#xf1 ; eiro^c ; Milagros Montes^c ; Sonia Pé ; rez Castro^d ; Jonathan Suá ; rez Ferná ; ndez^d ; Juan Garcí ; a Costa^e ; Mirian Ferná ; ndez^f ; Juan Carlos Galá ; n^g ; Marí ; a Teresa Cuevas^a ; Inmaculada Casas^a ; Pilar Pé ; rez Bre&#xf1 ; a^a ; Spanish Influenza Surveillance System (SISS)¹
• 关键词：Pandemic influenza A (H1N1) 2009 virus ; Haemagglutinin sequencing ; D222G substitution ; Severe respiratory infection
• 刊名：Journal of Clinical Virology
• 出版年：2011
• 出版时间：May 2011
• 年：2011
• 卷：51
• 期：1
• 页码：75-78
• 全文大小：132 K

文摘

Background

A change of aspartic acid (D) to glycine (G) at position 222 in the haemagglutinin (HA) protein of pandemic influenza A (H1N1) 2009 viruses was described in Norway on November 2009 with considerable frequency in fatal and severe cases. This change was detected in other countries and was related only with severe disease. Other substitutions to glutamic acid (E) or asparagine (N) at position 222 were detected among pandemic viruses but it is unclear what implications might have in terms of severity.

Objectives

To analyse the appearance of amino acid substitutions at position 222 in the HA protein of circulating viruses in Spain and to determine their relationships with the disease symptoms observed.

Study design

Pandemic influenza A (H1N1) 2009 viruses detected in respiratory samples of 273 severe and 533 non-severe cases from different Spanish regions were selected for sequencing of a partial segment of HA1 subunit and studied to monitor substitutions at position 222.

Results

D222G substitution was only detected in viruses from 14 severe cases (5.12 % ). D222E was found in viruses from 47 severe (17.21 % ) and from 52 non-severe cases (9.75 % ). D222N occurred in viruses from 3 additional severe cases (0.37 % ).

Conclusion

Appearance of D222G and D222E substitution in HA of pandemic influenza A (H1N1) viruses circulating in Spain might be related with severe respiratory disease.