S.?epidermidis strains from OIs (n?=?44) and healthy skin (HS; n?=?52) were isolated. The nor family genes were identified in CNS using PCR, sequencing and phylogenetic approaches. Nor family expression was determined by RT-PCR. NorA efflux activity was determined using the automated ethidium bromide method.
In-silico analysis showed that norA, mgrA/norR, and ?em>norB-like?and ?em>norC-like?(norB/norC) genes are present in CNS. The nor family genes were distributed and constitutively expressed in all S.?epidermidis strains studied. In one gatifloxacin resistant strain isolated from the endophthalmitis, treatment with gatifloxacin induced overexpression of the norA gene and resulted in high activity of NorA efflux.
These results indicate that the Nor family of proteins is present in CNS, and the NorA efflux mechanism for gatifloxacin response occurs in at least one strain of S.?epidermidis, contributing to gatifloxacin resistance.