Morphological and Genetical Variability among Rhizoctonia solani Isolates Causing Sheath Blight Disease of Rice

详细信息    查看全文

• 作者：Zakiah Rahman Moni^a ; Md Ansar Ali^b ; Md Shahidul Alam^c ; Md Asif Rahman^d ; Md Rejwan Bhuiyan^d ; ^e ; Md Salim Mian^d ; ^e ; Khandakar Md Iftekharuddaula^a ; Md Abdul Latif^d ; ^e ; Mohammad Ashik Iqbal Khan^d ; ^e ; ^{ashikjp@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：rice ; Rhizoctonia solani ; fungal variability ; molecular marker ; morphological character
• 刊名：Rice Science
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：23
• 期：1
• 页码：42-50
• 全文大小：1818 K

文摘

Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a reference isolate confirmed that all the isolates belonged to R. solani. Significant variation was observed in sclerotial size, shape and distribution. Un-weighted pair group method with arithmetic mean dendrogram constructed based on the Gower's general similarity coefficient showed that these isolates were grouped into four clusters at the 0.68 similarity coefficent according to morphological characters. Cluster I was a major cluster consisting of 13 isolates, while clusters II to IV consisted of 1 or 2 isolates. Analyses by variable number of tandem repeat and amplified fragment length polymorphism markers showed that the isolates were grouped into five and three clusters at a similarity coefficient of 0.64 and 0.69, respectively. Although most of the variability was found between isolates from different regions as expected, significant variation was observed within the isolates collected from similar agro-ecological regions. Our results suggest the presence of different races of R. solani within the same local geographic regions.