βA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development

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• 作者：Debasish Sinha ; Andrew Klise ; Yuri Sergeev ; Stacey Hose ; Imran A. Bhutto ; Laszlo Hackler Jr. ; Tanya Malpic-llanos ; Sonia Samtani ; Rhonda Grebe ; Morton F. Goldberg ; J. Fielding Hejtmancik ; Avindra Nath
• 关键词：β ; A3/A1-crystallin ; Astrocytes ; Retinal vasculature ; Remodeling ; Intermediate filaments ; Rat spontaneous mutation
• 刊名：Molecular and Cellular Neuroscience
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：37
• 期：1
• 页码：85-95
• 全文大小：2405 K

文摘

Vascular remodeling is a complex process critical to development of the mature vascular system. Astrocytes are known to be indispensable for initial formation of the retinal vasculature; our studies with the Nuc1 rat provide novel evidence that these cells are also essential in the retinal vascular remodeling process. Nuc1 is a spontaneous mutation in the Sprague–Dawley rat originally characterized by nuclear cataracts in the heterozygote and microphthalmia in the homozygote. We report here that the Nuc1 allele results from mutation of the βA3/A1-crystallin gene, which in the neural retina is expressed only in astrocytes. We demonstrate striking structural abnormalities in Nuc1 astrocytes with profound effects on the organization of intermediate filaments. While vessels form in the Nuc1 retina, the subsequent remodeling process required to provide a mature vascular network is deficient. Our data implicate βA3/A1-crystallin as an important regulatory factor mediating vascular patterning and remodeling in the retina.